Cytological Findings Suggesting Sexuality in Phytomonas davidi (Protozoa: Kinetoplastida)

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Isolation and characterization of kinetoplast DNA and RNA of Phytomonas davidi.

Kinetoplast DNA networks were isolated from stationary-phase culture forms of Phyromonas davidi. The networks banded in CsCl at a density of 1.699 g/ml and consisted of covalently closed circular molecules. The networks were sensitive to shear forces and exhibited several discrete sedimenting components in neutral and alkaline sucrose. Closed monomeric minicircles were isolated from sonicated n...

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Promastigotes of Leishmania donovani transformed into amastigotes and survived in cultured macrophages. Promastigotes of Leptomonas costoris, a kinetoplastid parasite of water striders, transformed into amastigotes but did not survive in cultured macrophages. A significant difference in size between the two species of parasitic protozoa allowed development of a bioassay in which the in vitro di...

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ژورنال

عنوان ژورنال: Memórias do Instituto Oswaldo Cruz

سال: 1997

ISSN: 0074-0276

DOI: 10.1590/s0074-02761997000200007